Detection of Amoebiasis by Using ELISA Technique among Patients in Duhok City

Authors- Muslim Abbas Allu, Hassan Mohsen Hassan

Abstract-In the present study, spanning from July 1, 2022, to December 31, 2022, fecal samples were procured from a cohort of 166 adult patients with symptoms of diarrhea Azadi Teaching Hospital of duhok city, Iraq Samples that exhibited positive results under microscopy were subjected to additional analysis using ELISA technique. The current data revealed that out of 166 stool samples examined with microscopy and iodine preparation, 53(31.92%) specimens were positive for E. histolytica trophozoites and cysts, while the remaining 113(68.08%) were negative for any amoebic stages. male was more infected than women. The percentage of specimens that were tested with E. histolytica ELISA Out of 166 stool specimens, 89(53.61%) were was from male, while the remaining specimens was from female 77(46.39%). The total of positive samples was 33 samples, while negative samples were 56 in males, while in females, the positive samples was 20 samples, while negative samples were 57 samples. The DRG stool ELISA revealed sensitivity and specificity (81.13%and 93.33%) respectively and predictive value of (97.87%).

DOI: /10.61463/ijset.vol.13.issue1.165