International Journal of Science, Engineering and TechnologyAuthors: Harisha. H, Shilpa M.P, Jyothi Hiremath, S. Shivaveerakumar
Abstract: Actinomycetes isolation, characterisation, and biotechnological uses, with a focus on Streptomyces species for the synthesis of extracellular tyrosinase. Using morphological, biochemical, and molecular criteria, actinomycetes were identified from samples taken from a variety of habitats, including freshwater, soil, and marine systems. Their capacity to produce enzymes, beneficial chemicals, and most notably melanin through copper-dependent tyrosinase activity is highlighted in the paper. Enzyme yield and melanin synthesis were maximized by optimizing fermentation and physicochemical conditions. Important discoveries include the discovery of strong Streptomyces isolates with high tyrosinase activity, which have uses in environmental remediation, bioprocessing, and biosensors. Techniques including qRT-PCR, SDS-PAGE, and UV-V is spectrophotometry confirmed the genetic expression, activity, and purification of tyrosinase. This study highlights the strain-specific adaptability of actinomycetes, indicating that more screening and genetic advancement will enable them to reach their full potential in industry and medicine. The complete examination of the attached files served as the foundation for all findings and conclusions, guaranteeing the incorporation of all pertinent information
DOI: http://doi.org/10.5281/zenodo.17357348
