Green Synthesis of Manganese Oxide and Copper Oxide Nanoparticles Using Piper Dravidii Leaves Extract and Evaluation of their Antimicrobial Activity

Authors- Yogita Shinde

Abstract-Manganese oxide nanoparticles (MnO₂-NPs) and copper oxide nanoparticles (CuO-NPs) have a wide range of advantageous features that make them ideal for a variety of biological applications when their surface chemistry is properly matched. These include targeted drug delivery, tissue regeneration, cell separation, hyperthermia therapy, and enhancing contrast in magnetic resonance imaging (MRI). The study successfully demonstrated an eco-friendly approach to synthesizing CuO-NPs and MnO₂-NPs using a previously unidentified extract derived from the Piper dravidii leaves. To characterize the synthesized nanoparticles, a comprehensive suite of analytical techniques was employed, including UV-visible spectrophotometry, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). Structural analysis revealed that the obtained MnO₂-NPs and CuO-NPs possessed a cubic morphology, were inherently stabilized without additional chemical modifications, and had particle sizes ranging between 26.98 nm and 65.20 nm. The phytochemicals present in the leaves extract functioned as natural reducing agents, playing a crucial role in the green synthesis of these nanoparticles. This environmentally sustainable process also contributed to their enhanced antibacterial properties. FT-IR analysis confirmed that the nanoparticles predominantly contained hydroxyl (-OH) and carboxyl (-COOH) functional groups, rendering them hydrophilic. Due to their innate surface chemistry, additional functionalization was unnecessary for their intended applications. To assess their antibacterial efficacy, the synthesized MnO₂-NPs and CuO-NPs were tested against both Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacterial strains. The results demonstrated notable antibacterial activity, with a zone of inhibition measuring 17 mm against E. coli and 15 mm against S. aureus. Given their natural stabilization, herbal attributes, and potent antimicrobial properties, these nanoparticles hold significant potential for diverse biomedical and biotechnological applications.

DOI: /10.61463/ijset.vol.12.issue6.956