International Journal of Science, Engineering and TechnologyAuthors: Shudanshu Sharma, Ravindra Kumar Jain
Abstract: Staphylococcus aureus is a well-known pathogen capable of producing enterotoxins during bacterial growth in contaminated milk, causing mastitis in dairy cattle and the ingestion of such preformed toxins is one of the major causes of food poisoning around the world. The capacity of S. aureus to produce biofilms increases its antibiotic resistance, posing a considerable challenge to dairy hygiene and public health. This research explored the antimicrobial activity of turmeric (Curcuma longa) extracts towards biofilm-producing S. aureus isolated from dairy milk. Milk samples were collected from dairy farms and screened to microbiological analysis for the isolation and identification of S. aureus by using selective culture media, morphological, and biochemical methods. The biofilm-forming ability of the isolates was determined by using Congo Red Agar method and the microtiter plate assay. Ethanolic turmeric extract was prepared using appropriate maceration extraction methods and tested for antimicrobial activity
